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tibodies, respectively. Cells were observed under a Zeiss LSM5 confocal microscope.doi: 10.1371/journal.pone.0073740.gmembrane (Figure 1B) [23]. A computer analysis could find no potential NLS throughout the C sequence (data not shown). First, to identify the amino acid region responsible for the nuclear localization of the Y1 protein, the subcellular localization of a series of Y1 mutants possess

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